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Cholesterol Esterase Activity of a Molecularly Imprinted Polymer

Author : J.-M. Kim*, K.-D. Ahn, G. Wulff

Journal : Macromolecular Chemistry and Physics, 202, 1105-1108

Publication Date : 2001-04-26

Papers : papers

Update Date : 16-07-20 02:08

Cholesterol Esterase Activity of a Molecularly.pdf (92.1K)[128]2016-07-20 02:08:29

A molecularly imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N,N′-diethyl(4-vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with K_m of 3.7 mM and k_cat of 2.2·10^–4 min^–1, respectively. A relative rate enhancement of 27-fold over the background buffer uncatalyzed reaction and of 2.4-fold in the presence of the control polymer were observed.

Link : http://onlinelibrary.wiley.com/doi/10.1002/1521-3935(20010401)202:7%3C1105::AID-MACP1105%3E3.0.CO;2-P/abstract

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Cholesterol Esterase Activity of a Molecularly Imprinted Polymer

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