Publication

Cholesterol Esterase Activity of a Molecularly Imprinted Polymer

Author : J.-M. Kim*, K.-D. Ahn, G. Wulff
Journal : Macromolecular Chemistry and Physics, 202, 1105-1108
Publication Date : 2001-04-26
Papers : papers
Update Date : 16-07-20 02:08
A molecularly imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N,N′-diethyl(4-vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with Km of 3.7 mM and kcat of 2.2·10–4 min–1, respectively. A relative rate enhancement of 27-fold over the background buffer uncatalyzed reaction and of 2.4-fold in the presence of the control polymer were observed.

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